路海,胡景霞,陈京,白波.吗啡持续刺激对小鼠树突状细胞发育及分泌IL-12的影响[J].济宁医学院学报,2011,(4):229-231,235 |
吗啡持续刺激对小鼠树突状细胞发育及分泌IL-12的影响 |
Effects of sustained morphine stimulation on dendritic cell development and IL-12 production |
投稿时间:2011-06-21 |
DOI:10.3969/j.issn.1000-9760.2011.04.001 |
中文关键词: 吗啡;树突状细胞;阿片受体;白介素-12 |
英文关键词: Morphine;Dendritic cell;Opioid receptor;IL-12 |
基金项目:国家自然科学基金项目(编号:30971081、30870932、81070961);山东省自然基金资助项目(编号:ZR2009DZ004) |
作者 | 单位 | E-mail | 路海 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | 胡景霞 | 泰山医学院, 山东泰安 271016 | | 陈京 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | 白波 | 济宁医学院神经生物学研究所, 山东济宁 272067 | bbai@mail.jnmc.edu.cn |
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中文摘要: |
目的 吗啡持续刺激对C57 BL/6小鼠骨髓来源的树突状细胞成熟的影响。方法 体外培养C57 BL/6小鼠骨髓来源的树突状细胞,培养过程中给予吗啡刺激,观察细胞形态的变化;应用酶联免疫吸附法检测树突状细胞分泌细胞因子IL-12水平的变化。结果 吗啡干预各组树突状细胞中细胞因子IL-12分泌水平较对照组显著升高。结论 吗啡持续刺激能够促进小鼠树突状细胞的成熟。 |
英文摘要: |
Objective To investigate the effects of sustained morphine stimulation on the bone marrow-derived dendritic cell(DC) maturation.Methods The primary C57BL/6 mouse bone marrow-derived dendritic cells(4-6weeks) were cultured in vitro.In course of cell culture morphine was added at the same time.The cell supernatants were collected at 7 days and interleukin-12(IL-12) levels were detected by enzyme-linked immunosorbent assay(ELISA).Results The results showed that the secretion of IL-12 in LPS+morphine groups was significantly increased compared with blank control group and LPS control group(P<0.01).But there was no difference between the LPS+morphine groups(P>0.05).Conclusion Sustained morphine stimulation on the mouse bone marrow-derived dendritic cells can promote dendritic cells to mature. |
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